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To demonstrate catalase in peroxisomes, Tetrahymena pyriformis
strain GL was incubated in an alkaline medium containing
3,3’-diaminobenzidine (DAB) and hydrogen peroxide
(H2O2) for 1, 2 and 4 hours. An electron-opaque
DAB reaction product is found after 2 (4) and 4 (5)
hours in unstained cells but not after 1 hour (3) of
incubation. On uranyl acetate and lead citrate stained thin sections
the peroxisomes but not mitochondria stain (6-8). Thus the
catalase is present only in the peroxisomes. EMs taken on 12/11/74 by
R. Allen with Hitachi HU11A TEM. Picture magnifications: Nos. 1-3,
25,000X; Nos. 6-7, 50,000X; No. 8, 75,000X. Bars = 0.25µm. Published
in J. Histochem. Cytochem. 23:599-606, 1975.
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