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01a-hal710312-29

Fig. 1a: section near left adoral surface

Halteria grandinella (obtained from Carolina Biological Supply Co., NC, U.S.A.) is an oligotrich ciliate. This cell lives in fresh water. It lacks long rows of somatic cilia called kineties but has a few somatic ciliary bristles as well as a well developed oral and adoral or perioral ciliature. The first set of 9 electron micrographs is of a serially sectioned cell fixed in collidine-buffered fixative. The sections are thicker than normal so that the EMs will appear less sharp when enlarged. Images for enlargement are not provided for the first 9 pictures. For a more complete study of this cell refer to Grain, Protistologica 8:179-197, 1972. Figure 1a is of a section near the cell’s left adoral surface where the contractile vacuole (cv) is located and where some of the oral ciliature are exposed in longitudinal section. A surface indentation may be near the CV pore (cv p), (also see Fig. 9 below.) One membranelle of the perioral ciliature is sectioned through its basal bodies. The cytosol contains condensed as well as expanded mitochondria (m). The uniformly round and electron transparent structures (pg) seen here appear filled with dense material under different fixation and staining conditions (see Fig. 2). These may be pigment granules (pg). EM taken on 3/12/71 by R. Allen with Hitachi HU11A TEM. Neg. 3,800X. Bar = 2µm.
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