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Paramecium caudatum exposed to the lectin Concanavalin A
conjugated to FITC after 5% paraformaldehyde or 10% formalin fixation
show the number and location of the nuclei in non permeabilized cells.
The nuclear envelopes become labeled. a) This species has one
fairly large micronucleus and a much larger macronucleus, both
enclosed by a nuclear envelope. The micronucleus tends to lie against
the macronucleus. b) During anaphase of micronuclear division
the micronucleus elongates within the intact nuclear envelope in the
anterior end of the cell. c) As the binary division furrow
begins to appear a very long division spindle forms. The macronucleus
also elongates. d) In late stages of binary fission the
daughter micronuclei separate from the division spindle and the two
ends of the elongated macronucleus separate and pinch off so that one
product of macronuclear division remains in the proter and the other
end remains in the opisthe. Fluorescent pictures taken by M. Aihara.
Bar for a-c = 25µm and for d = 40µm. Published in J. Protozool.
35:400-405, 1988.
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