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Quick-freeze deep-etch view of a radial arm showing a collecting
canal (cc) surrounded with smooth spongiome (ss) as well
as a few decorated tubules (ds). However several larger
vesicles lie next to the radial arm containing a lumen resembling the
contractile vacuole. These vesicles appear to be rounded decorated
tubules which have been transformed into vesicles or have not yet
tubulated and become part of the decorated spongiome of the mature
radial canal. Although we cannot be certain without further study we
believe this may represent a dividing cell as we do know that the
radial arms of the dividing cell, both their smooth and decorated
spongiomes, shorten as the new CVCs develop (Allen et al., J. Cell
Sci. 96:469-475, 1990; Fok et al., J. Eukaryot. Microbiol. 49:185-196,
2002 and see Fig. 32). The P-face of these vesicles
(Pds) has a large number of IMPs like that seen in
collecting canals. The highly porous E-face (Eds) of
several vesicles indicates that these vesicles are indeed products of
the decorated spongiome. EM taken on 6/22/88 by C. Schroeder with
Zeiss 10A TEM. Neg. 9,780X. Bar = 0.5µm.
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