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MAb DS-1 was then used to follow the development of new CVCs during
cell division and to document the retraction of the decorated
spongiome of the radial arms in the mature CVCs during division. This
was first correlated with division furrow formation (cytokinesis)
(Allen et al., J. Cell Sci. 96:469-475, 1990) and later by double
labeling with macronuclear division and with smooth spongiome changes
(Fok et al., J. Eukaryot. Microbiol. 49:185-196, 2002). New CVCs
develop anterior to both the CVC in the posterior (opisthe) and in the
anterior (proter) portions of the dividing cell. As the new CVCs form
the radial arms of the mature CVCs shorten until the new and old CVCs
are nearly the same size this occurs before the division furrow has
proceedfed far into the cell. By the time the daughter cells are
actually separated all four CVCs are fully functional. Lengthening of
the radial arms may continue throughout interphase as they are much
longer in cells ready to divide than in the daughter cells newly
separated. Pictures taken by M. Aihara. Published in J. Cell Sci.
96:469-475, 1990.
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