Home   Chapter 9

21-pmcvc27975

Fig. 21: the CV collapses into 40nm tubules

To determine what happens to the CV during systole we observed the CV in a living cell and then fixed the cell as soon as the CV had opened to the outside. It was apparent that the CV membrane does not flatten into an empty sac but the CV membrane undergoes rapid tubule formation and collapses as a continuous mat of tubules that maintains its connection to the microtubular ribbons (mt). These tubules are a uniform 40nm in diameter. EM taken on 6/9/96 by R. Allen with Zeiss 10A TEM. Neg. 19,800X. Bar = 0.2µm. Published in J. Exp. Biol. 200:1737-1744, 1997.
Download High Resolution TIF Image (4.1 MB)