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Chapter 9: Paramecium multimicronucleatum - Contractile Vacuole Complex

Video Of The Contractile Vacuole

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Fig. 1: whole filled contractile vacuole with CV pore
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Fig. 2: contractile vacuole in systole (empty) phase
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Fig. 3: contractile vacuole pore
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Fig. 4: grazing section through edge of CV pore
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Fig. 5: cross section of CVC in serial section 3 at the level of the pore
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Fig. 6: cross section of CVC in serial section 17
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Fig. 7: cross section of CVC in serial section 31
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Fig. 8: cross section of CVC in serial section 26
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Fig. 9: cross section of CVC in serial section 42
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Fig. 10: CV in diastole with radial canals open to CV
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Fig. 11: microtubular ribbons of CVC in cross section
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Fig. 12: collecting canal attachment to CV
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Fig. 13: sections of restricted ampullary necks near CV
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Fig. 14: one radial arm
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Fig. 15: collecting canal, smooth and decorated spongiomes
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Fig. 16: freeze fractured collecting canal
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Fig. 17: connection between smooth and decorated tubules
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Fig. 18: spongiomes back-filled with tannic acid
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Fig. 19: radial arm in cross section
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Fig. 20: collecting canal in diastole
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Fig. 21: the CV collapses into 40nm tubules
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Fig. 22: the CV membrane tubulates even in ruptured cells if systole occurs
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Fig. 23: collapse of CV membrane into tubules ~ perpendicular to ribbons
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Fig. 24: the smooth spongiome forms a 3-D array of cubic membrane
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Fig. 25: detail of smooth spongiome cubic membranes
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Fig. 26: QF-DE of cross fractured radial arm
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Fig. 27: thin section of decorated tubules
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Fig. 28: QF-DE of decorated tubules and collecting canal
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Fig. 29: drawing of CVC structures and CVC cycle
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Fig. 30: monoclonal antibody specific for decorated spongiome
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Fig. 31: development of CVCs
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Fig. 32: correlation of development of decorated with smooth spongiomes
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Fig. 33: immunogold labeling of decorated tubules
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Fig. 34: peripheral location of immunogold label on decorated tubules
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Fig. 35: comparing DS-1 label with a V-ATPase antibody raised to Dictyostelium
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Fig. 36: color image of the antigens A4 (green) and G4 (red) on the CVC of Paramecium
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Fig. 37: negatively stained and immunogold labeled V-ATPase
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Fig. 38: QF-DE of fracture faces of collecting canal
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Fig. 39: QF-DE of E-faces of collecting canal and smooth spongiome
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Fig. 40: QF-DE of P-face and lumen of ampulla
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Fig. 41: QF-DE of E-face of contractile vacuole
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Fig. 42: QF-DE of P-face of contractile vacuole
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Fig. 43: injection of DS-1 causes decorated tubules to vesiculate
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Fig. 44: QF-DE showing decorated tubule vesiculation
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Fig. 45: a model for membrane tubulation in Paramecium
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Fig. 46: Cross section of radial arm of CVC