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In 0.3 mM cytochalasin B (CB) treated cells the cytoproct
(cyp) remains open as this drug inhibits actin polymerization
and consequently the retrieval of spent vacuole membrane by tubulation
or vesiculation. In this case the cytoplasm, covered by an intact
membrane, bulges out through the open cytoproct. Normal membrane
retrieval had stopped and the network of actin, usually found around
the spent vacuole membrane at the open cytoproct, was absent. (For CB
study, see Allen and Fok, Eur. J. Cell Biol. 37:35-43, 1985). EM taken
on 9/24/82 by R. Allen with Hitachi HU11A TEM. Neg. 8,500X. Bar =
0.5µm. Published in Eur. J. Cell Biol. 37:35-43, 1985.
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