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When cells defecate a spent vacuole in the presence of horseradish
peroxidase (HRP) the HRP will enter the cavity of the cytoproct and
will be trapped in the small vesicles and tubules that are formed
during the retrieval of the vacuole’s membrane. When incubated in
H2O2 and diaminobenzidine an electron-opaque
reaction product will mark these vesicles. This allowed us to show
that these membrane remnants become bound to the cytopharyngeal
ribbons where they are transformed into discoidal vesicles and are
transported back to the cytopharynx for new digestive vacuole
formation (see Figures 21 and 22 under the section on the buccal
cavity and cytopharynx; also see Allen and Fok, J. Cell Sci.
45:131-145, 1980). cyp, cytoproct. EM taken on 2/23/82 by R.
Allen with Hitachi HU11A TEM. Neg. 11,750X. Bar = 0.5µm. Part
published in Adv. Cell Mol. Biol. Memb. 28:311-337, 1993.
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