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We raised a mAb to an antigen, A4, of the V-ATPase of P.
multimicronucleatum which is known to be particularly abundant in
the decorated spongiome of this cell’s contractile vacuole complex.
However, we also found that this antigen is present in moderate
amounts in the acidosome membrane (see Ishida et al., Protoplasma
196:12-20, 1997). Thus we conclude that acidosomes transfer V-ATPases
to the DV-II as they fuse with the phagosome. This is why the
digestive vacuole has a lower pH when it becomes a phagoacidosome (for
a study of vacuole pH in Paramecium see Fok, Lee and Allen, J.
Protozool. 29:409-414, 1982). EM taken on 2/10/93 by R. Allen with
Zeiss 10A TEM. Neg. 9,780X. Bar = 0.2µm. Published in Protoplasma
196:12-20, 1997.
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