|
To see if acidosomes contain hydrolases (digestive enzymes) we
incubated cells in Gomori’s medium which in the presence of acid
phosphatase (AcPase) and lead will form an electron-opaque
reaction product. Acidosomes (ac) surrounding a DV-I are free
of reaction product even though a nearby DV, presumably a DV-III,
contains abundant reaction product (AcPase). A few vesicles
assumed to be lysosomes (ly) also contain some reaction
product. This experiment demonstrated that acidosomes are not
lysosomes and do not contain digestive enzymes at least not AcPase. EM
taken on 4/8/83 by R. Allen with Hitachi HU11A TEM. Neg. 5,000X. Bar =
1µm.
|
