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| Monoclonal antibody, E30-2-1, was raised to antigen D6 that specifically labels acidosomes (ac) and DV-II. There was no label on the nascent vacuole membrane (nfv) or ciliary membranes (c). This cell had been incubated in 1.1µm latex beads for 3 minutes and fixed without a chase. There is some gold over the cytosol but the nascent vacuole lumen is essentially free of label. EM taken on 7/12/90 by R. Allen with Zeiss 10A TEM. Neg. 9,780X. Bar = 0.5µm. |
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