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Another mAb, B25-1-3, was raised to antigen Q2 (arbitrary code) to
the same set of membranes as mAb 132-F4-G6 labels. This mAb was more
reactive on frozen sections than the mAb in Figure 9. To tell the age
of vacuoles, cells were exposed to latex beads for 2.5 minutes and
then washed free of the beads (0.5 min) and then chased in bead-free
buffer for a known time before fixing. In this image the labeled
phagosome is a DV-I as it contained 1.1µm beads that were present in
the medium for the 3 minutes before the cells were fixed. Some
vesicles in the cytosol are also labeled but there is very little
nonspecific label over the general cytosol or the contents of the
vacuole. EM taken 10/24/92 by R. Allen with Zeiss 10A TEM. Neg.
19,800X. Bar = 0.2µm.
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