Monoclonal antibody for antigen B3 (called PC-2 in Eur. J. Cell Biol. 40:1-8, 1986) is located on a few digestive vacuoles in each cell from 1 min. to 25 min. in age. The ages of DVs were determined as in Fig. 8a and are 0-1 min. old in a/f (control cell) and b/g; 2-5 min.old in c/h; 6-9 min. old in d/i; and 22-25 min. old in e/j. The majority of DVs labeled with this mAb are 1-15 min old. Pictures taken by M. Aihara. Bar = 20µm. |
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