Quick-freeze deep-etch images show a more three dimensional view of
acidosomes surrounding a phagosome. Acidosomes are unique in that they
contain indentations of their membrane (arrowheads). The lumen
(luac) has a honeycombed appearance, also unique to
these vesicles. Their E-face (Eac) is almost free of
IMPs while their P-face (Pac) has many large
particles which leave pits in the E-face. Acidosomes maintain a close
association with the phagosome membrane. In this micrograph the
difference between the lumens of pretrichocyst vesicles (tric)
and acidosomes can be compared. Edv, E-face of
phagosome. EM taken on 5/26/92 by R. Allen with Zeiss 10A TEM. Neg.
9,780X. Bar = 0.5µm.
|