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In some cells exposed to horseradish peroxidase (HRP) for 30 sec and
then immediately fixed some HRP has already entered the cell by
endocytosis and has been removed from the early endosomes in carrier
vesicles. In this picture such carrier vesicles (cav) have
already docked at acidosomes (ac) that are themselves docked at
a nascent digestive vacuole (nfv). Carrier vesicles will fuse
with the acidosomes to add their content and membrane to the
acidosomes. This is why two populations of acidosomes are often seen
around the nascent phagosome and early DV-I, acidosomes that contain
HRP because they have fused with HRP-containing carrier vesicles and
those that have not fused with HRP-containing carrier vesicles. (See
Allen, Ma and Fok, J. Cell Sci. 106:411-422, 1993). EM taken on
2/24/82 by R. Allen with Hitachi HU11A TEM. Neg. 21,250X. Bar = 0.2µm.
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