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| a) Trichocysts labeled with the lectin Ulex europaeus (UEA I) agglutinin conjugated to FITC have only their tips labeled when cells are fixed in formalin. This lectin is specific for a-L-fucose in glycoproteins. Picture taken by M. Aihara. Bar = 10µm. b) When the cell was fixed in 5% paraformaldehyde the body of the trichocysts also had some UEA I-FITC fluorescence so that the body outlines were visible attached to the more heavily labeled tips. Picture taken by M. Aihara. Bar = 10µm. |
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