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A favorable view of one trichocyst docking site in a quick-freeze
deep-etch image shows the prominent outer ring of 37 IMPs and about
the same number of IMPs in the inner ring more deeply embedded in the
cytosolic leaflet. Pits, where the rosette was removed during
fracturing of the chemically-unfixed rapid-frozen cell, are seen at
the center of the docking site. Two gaps in the rings (arrows)
indicate the site of the septum in the underlying alveoli (see Fig. 2)
where the plasma membrane is not linked to the alveolar rims by the
ring IMPs. EM taken on 2/15/90 by R. Allen with Zeiss 10A TEM. Neg.
31,500X. Bar = 0.2µm. Inset: The E-face of the rapidly-frozen chemically-unfixed plasma membrane retains the rosette of 8 or 9 IMPs that form part of a docking mechanism for the tip of the trichocyst membrane. EM taken on 2/15/90 by R. Allen with Zeiss 10A TEM. Neg. 12,000X. Bar = 50nm. |
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