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In quick-freeze deep-etch preparations in which cells are slam frozen
the pellicle and the plasma membrane tends to be flattened. The double
rings (arrowheads) are present but only the outer ring of IMPs
stand out prominently. The inside ring seems embedded more deeply into
the plasma membrane’s P-face, i.e., into the cytosolic leaflet of this
membrane. Three sets of rings are seen in this plate along with a
parenthesis-shaped ring (arrow) which is present at a docking
site lacking a trichocyst. Pits appear in the rings where the rosettes
were removed that remained attached to the E-face of the plasma
membrane. EM taken on 3/6/90 by R. Allen with Zeiss 10A TEM. Neg.
19,800X. Bar = 0.2µm.
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