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Fig. 4: freeze fracture of plasma membrane-trichocyst fusion sites

A freeze fracture view of the P-face of the plasma membrane in which the underlying alveoli are expanded. The fractured off cilia occupy the bases of the surface depressions. IMP double rings lie between the cilia (arrowheads) in each kinetal (ciliary) row. A rosette of about 8 IMPs forms a circle in the center of the double ring. This rosette is linked to the trichocyst membrane (see Fig. 2) at the trichocyst’s tip. The double rings are linked to the underlying edges of the alveoli that form the septum through which the trichocyst passes to dock at the plasma membrane. Normally trichocysts are aligned only along the row of cilia, however, in this image extra trichocyst fusion sites (arrows) are found to the sides of the ciliary rows. EM taken on 6/4/73 by R. Allen with Hitachi HU11A TEM. Neg. 10,250X. Bar = 0.5µm.
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