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The first culture of P. multimicronucleatum which I had
obtained from Carolina Biological Supply House contained a bacterial
endosymbiont that resided specifically in the space between the two
membranes of the nuclear envelope or in outpocketings of this space so
that the bacteria were enclosed by the outer membrane of the nuclear
envelope. Such endosymbionts have been observed before (Jenkins, J.
Gen. Microbiol. 61:355-359, 1970). In this micrograph the bacteria
(bact) are associated with the micronucleus, which has a
remnant bundle of fibers (arrowheads) and microtubules, but
bacteria are also associated with the macronucleus (not shown). EM
taken on 12/9/70 by R. Allen with Hitachi HU11A TEM. Neg. 19,500X. Bar
= 0.2µm.
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