|
Usually the four micronuclei in P. multimiucronucleatum are
scattered throughout the anterior end of the cell, however, in this
cell taken from an experiment in which the cells were exposed to HRP
for 3 seconds and then chased in medium without HRP for 3 minutes, a
cell was observed with four micronuclei in the same area. These
micronuclei have a typical vesicular interphase appearance with a
central electron opaque mass except for the lowest micronucleus which
was sectioned at its edge. Micronuclei appear to have a
circumferential band of material running around the inside of the
nucleus (arrowheads) reminding one of the band of microtubules
in nucleated red blood cells of fish and birds that help maintain
their unique flattened shape. Here the micronuclei appear to be
elongated somewhat although they do not appear to be stretched out
into a strongly elliptical shape. Each micronucleus is surrounded by a
typical nuclear envelope. EM taken on 3/18/81 by R. Allen with Hitachi
HU11A TEM. Neg. 10,250X. Bar = 0.5µm. Published in Paramecium, H.-D.
Gortz, ed., Springer-Verlag, Berlin, p.34, 1988.
|
