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30-pm

Fig. 30: immunofluorescent images of striated bands of two species of Paramecium

We raised a monoclonal antibody to the striated bands of P. multimicronucleatum which is shown in the fluorescent micrographs in Figures 9 and 10. The pattern of this cytoskeletal meshwork is different from the infraciliary lattice shown in Fig. 26. The sites of basal bodies and cilia are unlabeled and longitudinal rows of these sites correspond to the kineties. The striated bands form a layer under the longitudinal and lateral ridges of the pellicle. Contractile vacuole pores appear in Fig. 9a (arrows) as unlabeled, areas and the anterior suture along the ventral surface is apparent in Fig. 9b (arrow). Fig. 10a-b are of P. tetraurelia which reacts with the same mAb demonstrating that striated bands are also present in this species. arrowhead, cytoproct; o, oral region. Micrographs taken by M. Aihara. Published in J. Euk. Microbiol. 45:202-209, 1998.
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