BIOGRAPHICAL SKETCH

NAME TITLE SEX
     
Richard D. Allen Professor of Microbiology Male

 

EDUCATION

INSTITUTION AND LOCATION DEGREE YEAR CONFERRED SCIENTIFIC FIELD
       
Greenville College , Greenville, IL B.A. 1957 Biology
University of Illinois, Urbana, IL M.S. 1960 Botany
Iowa State University, Ames, Iowa Ph.D. 1964 Cell Biology
Harvard University, Cambridge, Mass. Postdoc 1964-1965 Cell Biology

 

RESEARCH AND/OR PROFESSIONAL EXPERIENCE

Academic Positions and Directorship

  1. PHS Predoctoral Fellowship, 1962-1964 and University of Illinois Fellowship, 1959-1960.
  2. PHS Postdoctoral Fellowship (with Prof. K.R. Porter), Harvard, 1964-1965.
  3. Assistant Professor of Biology, Messiah College, Grantham, Pa, 1965-1968.
  4. Director of the EM Service Laboratory, Associate in Electron Microscopy and Lecturer in Biology, Harvard University, 1968-1969.
  5. Associate Professor of Microbiology, University of Hawaii, Honolulu, 1969-1975.
  6. Visiting Professor, University of Colorado, Boulder, 1975-1976.
  7. Professor of Microbiology, University of Hawaii, Honolulu, 1975-present.
  8. Member of the Graduate Faculty, Department of Tropical Medicine, University of Hawaii, 1977-present, and CMNS Graduate Faculty 1988-present.
  9. Program Director, University of Hawaii Biomedical Research Support Grant, NIH, 1982-1986.
  10. Director, Biological EM Facility, University of Hawaii, 1985-present.

Editorial Positions

  1. Board of Reviewers, for J. Histochem. Cytochem., 1975-1981; J. Protozool., 1975-1979 and 1981-1986; and Eur. J. Cell Biol., 1979-1984.
  2. Editor, Protoplasma, 1990 - 2000.

Professional Society Memberships and Honors

  1. AAAS 1964 - present; Fellow, 1988.
  2. Society of Protozoologists 1967 - present; Seymour Hutner Prize, 1978; President, 1981; Chairman, Nomination Committee, 1986.
  3. American Society Cell Biology, 1969 - present.
  4. Sigma Xi 1964 - present.

 

Research Grant Support

  1. National Institutes of Health grant - GM-17991 entitled "Microtubular Systems", 1972 to 1978. Total Direct Costs $174,176.
  2. American Heart Association Grant-In-Aid - 76990 entitled "Control of Contractility in Primitive Motile Systems", 1976 to 1978. Total Direct Costs $24,380.
  3. National Science Foundation - PCM 78-15893 entitled "Membrane Dynamics in Intracellular Digestion", 1979 to 1982. Total Direct and Indirect Costs $165,000.
  4. National Institutes of Health - GM-27283 entitled "Cellular Membrane Recycling", 1980 to 1982. Total Direct Costs $40,067.
  5. National Science Foundation - PCM 81-10802 entitled "Membrane Dynamics in Intracellular digestion", 1981 to 1984. Total direct and Indirect Costs $175,000.
  6. National Science Foundation - PCM 82-01700 entitled "Cellular Membrane Recycling", 1982 to 1985. (Co-P.I. with Dr. A.K. Fok, P.I.). Total Direct and Indirect Costs $105,000.
  7. National Science Foundation - PCM 82-12316 entitled "Acquisition of a Transmission Electron Microscope", 1983 to 1984. Total Direct Costs $110,000. Matching money from U.H., $55,000.
  8. National Science Foundation - PCM 84-02881 entitled "Membrane Dynamics in Intracellular Digestion", 1984 to 1987. (Co-P.I. with Dr. A.K. Fok, P.I.). Total Direct and Indirect Costs $180,000.
  9. National Science Foundation - PCM 84-00418 entitled "Purchase of Freeze-etch Equipment", 1984 to 1985. Total Direct Costs $60,000. Matching money from U.H., $30,000.
  10. National Science Foundation - DCB 85-02212 entitled "Membrane Traffic: Monoclonal Antibody Study", 1985 to 1988. Total Direct and Indirect Costs $261,000.
  11. National Science Foundation - BBS-8721751 entitled "Cryoelectron Microscopy Equipment", 1988 to 1990. Total Direct Costs $24,330. Matching money from U.H., $12,165.
  12. National Science Foundation - DCB 87-18598 entitled "Membrane Dynamics in Intracellular Digestion", 1988 to 1991. (Co-P.I. with Dr. A.K. Fok, P.I.). Total Direct and Indirect costs $181,012.
  13. National Science Foundation - DCB 88-19182 entitled "Membrane Traffic and Topography", 1989 to 1992. Total Direct and Indirect Costs $244,000.
  14. National Science Foundation - DIR 88-20761 entitled "Acquisition of a High Resolution Scanning Electron Microscope", 1989 to 1991. Total Direct Costs $110,000. Matching money from U.H., $55,000.
  15. National Science Foundation - DCB 90-17455 entitled "Regulation of Microtubule-based Vesicle Transport", 1991 to 1993, (Co-P.I. with Dr. A.K. Fok, P.I.). Total Direct and Indirect Costs $298,000.
  16. National Science Foundation - MCB 92-06097 entitled "The Endosome System and Membrane Trafficking", 1992 to 1995. Total Direct and Indirect Costs $300,000.
  17. National Science Foundation - MCB 95-05910 entitled "Osmoregulation and Contractile Vacuole Function", 1995-1998. Total Direct and Indirect Costs $345,000.
  18. National Science Foundation - MCB 98-09929 entitled "Osmoregulation and Contractile Vacuole Function", 1998-2001. Total Direct and Indirect Costs $368,717.
  19. Department of Defense HBCU/MI Infrastructure Support Program for Research Instrumentation - Department of the Army Grant No. DAAG55-98-1-0352 entitled "Transmission Electron Microscope for Biological Research". 1 June l998 - 31 May 1999. $322,338. Matching Money from U.H., $161,000.
  20. National Science Foundation - MCB 01-36362 entitled Osmoregulation and Contractile Vacuole Dynamics", 2002-2005, Total Direct and Indirect Costs $376,000.

PUBLICATIONS

  1. Allen, R.D. and C.C. Bowen. 1966. Fine structure of Psilotum nudum cells during division. Caryologia. 19:229-342.
  2. Allen, R.D. 1967. Fine structure, reconstruction and possible functions of various components of the cortex of Tetrahymena pyriformis. J. Protozool. 14:553-565.
  3. Allen, R.D. 1968. A reinvestigation of cross-sections of cilia. J. Cell Biol. 37:825-831.
  4. Allen, R.D. 1969. The morphogenesis of basal bodies and accessory structures of the cortex of the ciliated protozoan, Tetrahymena pyriformis. J. Cell Biol. 40:716-733.
  5. Allen, R.D. 1971. Fine structure of membranous and microfibrillar systems in the cortex of Paramecium caudatum. J. Cell Biol. 49:1-20.
  6. Allen, R.D. and P. Baumann. 1971. Structure and arrangement of flagella in species of the genus Beneckea and Photobacterium fischeri. J. Bacteriol. 107:295-302.
  7. Baumann, P., L. Baumann, M. Mandel and R.D. Allen. 1971. Taxonomy of marine bacteria: Beneckea nigrapulchrituda sp. n. J. Bacteriol. 108:1380-1383.
  8. Baumann, L., P. Baumann, M. Mandel and R.D. Allen. 1972. Taxonomy of aerobic marine eubacteria. J. Bacteriol. 110:402-429.
  9. Allen, R.D. 1973. Structures linking the myonemes, endoplasmic reticulum and surface membranes in the contractile ciliate Vorticella. J. Cell Biol. 56:559-579.
  10. Allen, R.D. 1973. Contractility and its control in peritrich ciliates. J. Protozool. 20:25-36.
  11. Allen, R.D. and R.W. Wolf. 1974. The cytoproct of Paramecium caudatum: structure and function, microtubules and fate of food vacuole membranes. J. Cell Sci. 14:611-631.
  12. Taylor, V.I., P. Baumann, J.L. Reichelt and R.D. Allen. 1974. Isolation, enumeration, and host range of marine Bdellovibrios. Archiv fur Mikrobiologie. 98:101-114.
  13. Allen, R.D. 1974. Food vacuole membrane growth with microtubule-associated membrane transport in Paramecium. J. Cell Biol. 63:904-922.
  14. Allen, R.D. 1975. Evidence for firm linkages between microtubules and membrane-bounded vesicles. J. Cell Biol. 64:497-503.
  15. Folsome, C.E., R.D. Allen and N. Ichinose. 1975. Organic microstructures as products of Miller-Urey electrical discharges. Precambrian Research. 2:263-275.
  16. Fok, A.K. and R.D. Allen. 1975. Cytochemical localization of peroxisomes in Tetrahymena pyriformis. J. Histochem. Cytochem. 23:599-606.
  17. Allen, R.D. and K. Hausmann. 1976. Membrane behavior of exocytic vesicles. I. The ultrastructure of Paramecium trichocysts in freeze-fracture preparations. J. Ultrastruct. Res. 54:224-234.
  18. Hausmann, K. and R.D. Allen. 1976. Membrane behavior of exocytic vesicles. II. Fate of the trichocyst membranes in Paramecium after induced trichocyst discharge. J. Cell Biol. 69:313-326.
  19. Allen, R.D. 1976. Freeze-fracture evidence for intramembrane changes accompanying membrane recycling in Paramecium. Cytobiologie. 12:254-273.
  20. Hausmann, K. and R.D. Allen. 1977. Membranes and microtubules of the excretory apparatus of Paramecium caudatum. Cytobiologie. 15:303-320.
  21. Allen, R.D. 1978. Membranes of ciliates: ultrastructure, biochemistry and fusion. In Membrane Fusion, Poste, G. and G.L. Nicolson (eds.) Elsevier/North Holland, Amsterdam, pp. 657-763.
  22. Allen, R.D. 1978. Particle arrays in the surface membranes of Paramecium: junctional and possible sensory sites. J. Ultrastruct. Res. 63:64-78.
  23. Allen, R.D. and R.W. Wolf. 1978. Membrane recycling at the cytoproct of Tetrahymena. J. Cell Sci. 35:217-227.
  24. Fok, A.K. and R.D. Allen. 1979. Axenic Paramecium caudatum. I. Mass culture and structure. J. Protozool. 26:463-470.
  25. Allen, R.D. and A.K. Fok. 1980. Membrane recycling and endocytosis in Paramecium confirmed by horseradish peroxidase pulse-chase studies. J. Cell Sci. 45:131-145.
  26. Allen, R.D. and L.A. Staehelin. 1981. Digestive system membranes: freeze-fracture evidence for differentiation and flow in Paramecium. J. Cell Biol. 89:9-20.
  27. Fok, A.K. and R.D. Allen. 1981. Axenic Paramecium caudatum. II. Changes in fine structure with culture age. Eur. J. Cell Biol. 25:182-192.
  28. Fok, A.K., R.D. Allen and E.S. Kaneshiro. 1981. Axenic Paramecium caudatum. III. Biochemical and physiological changes with culture age. Eur. J. Cell Biol. 25:193-201.
  29. Fok, A.K., Y. Lee and R.D. Allen. 1982. The correlation of digestive vacuole pH and size with digestive cycle in Paramecium caudatum. J. Protozool. 29:409-414.
  30. Leung, K.P. and R.D. Allen. 1982. Phagosomal-phagolysosomal membrane dynamics of stimulated mouse peritoneal macrophages. Eur. J. Cell Biol. 29:1-12.
  31. Allen, R.D. and A.K. Fok. 1983. Phagosome fusion vesicles of Paramecium. I. Thin-section morphology. Eur. J. Cell Biol. 29:150-158.
  32. Allen, R.D. and A.K. Fok. 1983. Phagosome fusion vesicles of Paramecium. II. Freeze-fracture evidence for membrane replacement. Eur. J. Cell Biol. 29:159-165.
  33. Allen, R.D. and A.K. Fok. 1983. Nonlysosomal vesicles (acidosomes) are involved in phagosome acidification in Paramecium. J. Cell Biol. 97:566-570.
  34. Allen, R.D. 1984. Paramecium phagosome membrane: from oral region to cytoproct and back again. J. Protozool. 31:1-6.
  35. Fok, A.K., J.H. Muraoka and R.D. Allen. 1984. Acid phosphatase in the digestive vacuoles and lysosomes of Paramecium caudatum: a timed study. J. Protozool. 31:216-220.
  36. Fok, A.K., S.S.-K. Leung and R.D. Allen. 1984. Modulation of the digestive-lysosomal system in Paramecium caudatum. I. Effects of temperature. Eur. J. Cell Biol. 34:265-270.
  37. Allen, R.D. and A.K. Fok. 1984. Membrane behavior of exocytic vesicles. III. Flow of horseradish-peroxidase labeled trichocyst membrane remnants in Paramecium. Eur. J. Cell Biol. 35:27-34.
  38. Allen, R.D. and A.K. Fok. 1984. Stages of digestive vacuoles in Paramecium: membrane surface differences and location. Eur. J. Cell Biol. 35:149-155.
  39. Allen, R.D. and A.K. Fok. 1984. Retrieval of lysosomal membrane and acid phosphatase from phagolysosomes of Paramecium caudatum. J. Cell Biol. 99:1955-1959.
  40. Fok, A.K., S.S.-K. Leung, D.P. Chun and R.D. Allen. 1985. Modulation of the digestive lysosomal system in Paramecium caudatum. II. Physiological effects of cytochalasin B, colchicine, trifluoperazine and compound 48/80. Eur. J. Cell Biol. 37:27-34.
  41. Allen, R.D. and A.K. Fok. 1985. Modulation of the digestive-lysosomal system in Paramecium caudatum. III. Morphological effects of cytochalasin B. Eur. J. Cell Biol. 37:35-43.
  42. Fok, A.K., M.S. Ueno and R.D. Allen. 1986. Differentiation of Paramecium phagosome membrane and stages using monoclonal antibodies. Eur. J. Cell Biol. 40:1-8.
  43. Fok, A.K., M.S. Ueno, E.A. Azada and R.D. Allen. 1987. Phagosomal acidification in Paramecium: effects on lysosomal fusion. Eur. J. Cell Biol. 43:412-420.
  44. Allen, R.D. 1988. Cytology, chapter in book Paramecium, H.-D. Görtz, ed. Springer-Verlag, Berlin. pp. 4-40.
  45. Fok, A.K. and R.D. Allen. 1988. The lysosome system, chapter in book Paramecium, H.-D. Görtz, ed. Springer-Verlag, Berlin. pp. 301-324.
  46. Allen, R.D. and A.K. Fok. 1988. Membrane dynamics of the contractile vacuole complex of Paramecium. J. Protozool. 35:63-71.
  47. Hausmann, K., A.K. Fok and R.D. Allen. 1988. Immunocytochemical analysis of trichocyst structure and development in Paramecium. J. Ultrastruct. Mol. Struct. Res. 99:213-225. (Cover)
  48. Fok, A.K., B.C. Sison, Jr., M.S. Ueno and R.D. Allen. 1988. Phagosome formation in Paramecium: effects of solid particles. J. Cell Sci. 90:517-524.
  49. Allen, R.D., M.S. Ueno and A.K. Fok. 1988. A survey of lectin binding in Paramecium. J. Protozool. 35:400-407.
  50. Fok, A.K., R.P. Olegario, M.S. Ueno and R.D. Allen. 1988. Characterization of monoclonal antibodies to trichocyst antigens in Paramecium. J. Cell Sci. 91:191-199.
  51. Allen, R.D., C.C. Schroeder and A.K. Fok. 1989. Intracellular binding of wheat germ agglutinin by Golgi complexes, phagosomes and lysosomes of Paramecium multimicronucleatum. J. Histochem. Cytochem. 37:195-202.
  52. Allen, R.D., C.C. Schroeder and A.K. Fok. 1989. An investigation of mitochondrial inner membranes by rapid-freeze and deep-etch techniques. J. Cell Biol. 108:2233-2240. (Cover)
  53. Allen, R.D., C.C. Schroeder and A.K. Fok. 1989. Phagosome-lysosome membrane traffic in Paramecium. Proceedings of Workshop on Protein Traffic, ILRAD, Nairobi, Kenya, Aug. 30 - Sept. 2, 1988, pp. 12-17.
  54. Fok, A.K., M.S. Ueno and R.D. Allen. 1989. Control of intracellular digestion in Paramecium. Proceedings of Workshop on Protein Traffic, ILRAD, Nairobi, Kenya, Aug. 30 - Sept. 2, 1988, pp. 45-59.
  55. Cole, T.A., A.K. Fok, M.S. Ueno and R.D. Allen. 1990. Use of nile red as a rapid measure of lipid content in ciliates. Eur. J. Protistol. 25:361-368.
  56. Allen, R.D., M.S. Ueno, L.W. Pollard and A.K. Fok. 1990. Monoclonal antibody study of the decorated spongiome of contractile vacuole complexes of Paramecium. J. Cell Sci., 96:469-475. (Cover)
  57. Fok, A.K. and R.D. Allen. 1990. The phagosome-lysosome membrane system and its regulation in Paramecium. International Review of Cytology, 123:61-94.
  58. Schroeder, C.C., A.K. Fok and R.D. Allen. 1990. Vesicle transport along microtubular ribbons and cytoplasmic dynein isolation from Paramecium. J. Cell Biol. 111:2553-2562.
  59. Allen, R.D., C. C. Schroeder and A.K. Fok. 1992. Endosomal system of Paramecium: coated pits to early endosomes. J. Cell Sci. 101:449-461.
  60. Allen, R.D. and A.K. Fok. 1993. Endosomal membrane traffic of ciliates. In Membrane Traffic in Protozoa, H. Plattner ed., JAI Press Inc., Greenwich, Conn. Part B:283-309.
  61. Fok, A.K. and R.D. Allen. 1993. Membrane flow in the digestive cycle in Paramecium. In Membrane Traffic in Protozoa, H. Plattner ed. JAI Press Inc., Greenwich, Conn. Part B:311-337.
  62. Allen, R.D., L. Ma and A.K. Fok. 1993. Acidosomes: recipients of multiple sources of membrane and cargo during development and maturation. J. Cell Sci. 106:411-422.
  63. Allen, R.D. and A.K. Fok. 1993. Non-clathrin vesicle coats and filament networks in the transition zone and trans-Golgi region of the Golgi complex of Paramecium. J. Struct Biol. 110:215-226.
  64. Ishida, M., M.S. Aihara, R.D. Allen and A.K. Fok. 1993. Osmoregulation in Paramecium: the locus of fluid segregation in the contractile vacuole complex. J. Cell Sci. 106:693-702.
  65. Fok, A.K., M. Clarke, L. Ma and R.D. Allen. 1993. Vacuolar H+-ATPase of Dictyostelium discoideum: A monoclonal antibody study. J. Cell Sci. 106:1103-1113.
  66. Sturgill-Koszycki, S., P.H. Schlesinger, P. Chakraborty, P.L. Haddix, H.L. Collins, A.K. Fok, R.D. Allen, S. Gluck, J. Heuser and D.G. Russell. 1994. Lack of acidification in Mycobacterium phagosomes produced by exclusion of the vesicular proton-ATPase. Science 263:678-681. (Cover)
  67. Dohra, H., M. Fujishima, A.K. Fok and R.D. Allen. 1994. Monoclonal antibody to a bacterial endonuclear symbiont Holospora cross-reacts with proteins of contractile vacuole radial canals of Paramecium species. J. Eukaryotic Micro. 41:503-510.
  68. Allen, R.D. and R.K. Peck. 1994. Intracellular Traffic. in K. Hausmann and N. Hulsmann (eds.), Progress in Protozoology, pp. 105-111, Gustav Fischer Verlag, Stuttgart.
  69. Fok, A.K., H. Wang, A. Katayama, M.S. Aihara and R.D. Allen. 1994. 22S axonemal dynein is preassembled and functional prior to being transported to and attached on the axonemes. Cell Motil. Cytoskeleton. 29:215-224.
  70. Kathariou, S., C. Mizumoto, R.D. Allen, A.K. Fok, N. Promadej and A.A. Benedict. 1994. Monoclonal antibodies specific for serotype 4b Listeria monocytogenes. Appl. Environ. Microbiol. 60:3548-3552.
  71. Allen, R.D., N.P. Bala, R.F. Ali, D.M. Nishida, M.S. Aihara, M. Ishida and A.K. Fok. 1995. Rapid bulk replacement of acceptor membrane by donor membrane during phagosome to phagoacidosome transformation in Paramecium. J. Cell Sci., 108:1263-1274.
  72. Kathariou, S., R, Kanenaka, R.D. Allen, A.K. Fok and C. Mizumoto. 1995. Repression of motility and flagellin production is stronger in Listeria monocytogenes than in the nonpathogenic species Listeria innocua. Can. J. Micro., 41: 572-577.
  73. Allen, R.D. 1995. Intracellular Membrane Trafficking in Paramecium. Proceedings of The 4th Asian Conference on Ciliate Biology and The International Symposium on Cell Motility and Cytogenesis. Waseda University, Tokyo, Japan. March 27-31, 1995., pp 91-99.
  74. Fok, A.K., M.S. Aihara, M. Ishida, K.V. Nolta, T.L. Steck and R.D. Allen. 1995. The pegs on the decorated tubules of the contractile vacuole complex of Paramecium are proton pumps. J. Cell Sci., 108:3163- 3170.
  75. Allen, R.D. 1995. Membrane tubulation and proton pumps. Protoplasma 189:l-8. New ideas paper.
  76. Ishida, M., A.K. Fok, M.S. Aihara, and R.D. Allen, 1996. Hyperosmotic stress leads to reversible dissociation of the proton pump-bearing tubules form the contractile vacuole complex in Paramecium. J. Cell Sci. 109:229-237.
  77. Fujishima, M., M. Iwamoto, A.K. Fok and R.D. Allen. 1996. A macronuclear nucleoli-specific protein of the ciliate Paramecium caudatum. Eur. J. Protistol. 32:25-31.
  78. Fok, A.K., L. Ma, M.S. Aihara and R.D. Allen. 1996. High resolution view of the true cytosolic membrane surface of phagosomes of known ages purified from Paramecium. Eur. J. Cell Biol. 71:259-269.
  79. Naitoh, Y., T. Tominaga, M. Ishida, A.K. Fok, M.S. Aihara and R.D. Allen. 1997. How does the contractile vacuole of Paramecium expel fluid? Modelling the expulsion mechanism. J. Exp. Biol. 200:713-721. (Cover)
  80. Ishida, M., M.S. Aihara, R D. Allen and A.K. Fok. 1997. Acidification of the young phagosomes of Paramecium is mediated by proton pumps derived from the acidosomes. Protoplasma 196:12-20.
  81. Naitoh, Y., T. Tominaga, R.D. Allen. 1997. The contractile vacuole fluid discharge rate is determined by the vacuole size immediately before the start of discharge in Paramecium multimicronucleatum. J. Exp. Biol. 200:1737-1744.
  82. Allen, R.D., M.S. Aihara and A.K. Fok. 1998. The striated bands of Paramecium are immunologically distinct from the centrin-specific infraciliary lattice and cytostomal cord. J. Eukaryot. Microbiol. 45:202-209.
  83. Tominaga, T., R.D. Allen and Y. Naitoh. 1998. Electrophysiology of the in situ contractile vacuole complex of Paramecium reveals its membrane dynamics and electrogenic site during osmoregulatory activity. J. Exp. Biol. 201:451-460. (Cover)
  84. Tominaga, T., R.D. Allen and Y. Naitoh. 1998. Cyclic changes in the tension of the contractile vacuole complex membrane control its exocytotic cycle. J. Exp. Biol. 201:2647-2658. (Cover)
  85. Yamauchi, K., M.S. Aihara, M. Ishida, R.D. Allen and A.K. Fok. 1999. Cloning and sequencing of a protein involved in phagosomal membrane fusion in Paramecium. Mol. Biol. Cell 10:1031-1041.
  86. Tominaga, T., Y. Naitoh and R. D. Allen. 1999. A key function of non-planar membranes and their associated microtubular ribbons in contractile vacuole membrane dynamics is revealed by electrophysiologically controlled fixation of Paramecium. J. Cell Sci. 112:3733-3745.
  87. Flanary, P.L., R.D. Allen, L. Dons and S. Kathariou. 1999. Insertional inactivation of the Listeria monocytogenes cheYA operon abolishes response to oxygen gradients and reduces the number of flagella. Can. J. Microbiol. 45:646-652.
  88. Tani, T., R.D. Allen and Y. Naitoh. 2000. Periodic tension development in the membrane of the in vitro contractile vacuole of Paramecium: modification by bisection, fusion and suction. J. Exp. Biol. 203:239-251.
  89. Allen, R.D. and A.K. Fok. 2000. Membrane trafficking and processing in Paramecium. Int. Rev. Cytol. 198:277-318. Review. (Cover)
  90. Allen, R.D. 2000. The contractile vacuole and its membrane dynamics. BioEssays 22:1035-1042. Feature article. (Cover)
  91. Stock, C., R.D. Allen and Y. Naitoh. 2001. How external osmolarity affects the activity of the contractile vacuole complex, the cytosolic osmolarity and the water permeability of the plasma membrane in Paramecium multimicronucleatum. J. Exp. Biol. 204:291-304. .
  92. Tani, T., R.D. Allen and Y. Naitoh. 2001. Cellular membranes that undergo cyclic changes in tension: direct measurement of force generation by an in vitro contractile vacuole of Paramecium multimicronucleatum. J. Cell Sci. 114:785-795.
  93. Ishida, M., R.D. Allen and A.K. Fok. 2001. Phagosome formation in Paramecium: roles of somatic and oral cilia and of solid particles as revealed by video microscopy. J. Eukaryot. Microbiol. 48:640:646.
  94. Allen, R.D. and Y. Naitoh. 2002. Osmoregulation and contractile vacuoles of protozoa. In Molecular Mechanisms of Water Transport across Biological Membranes, T. Zeuthen and W. D. Stein, eds. Special edition of Int. Rev. Cytol., 215:351-394. Review.
  95. Fok, A.K., K. Yamauchi, A. Ishihara, M.S. Aihara, M. Ishida and R.D. Allen. 2002. The vacuolar-ATPase of Paramecium multimicronucleatum: gene structure of the B subunit and the dynamics of the V-ATPase-rich osmoregulatory membranes. J. Eukaryot. Microbiol., 49:185-196.
  96. Stock, C., H.K. Grønlien, R.D. Allen and Y. Naitoh. 2002. Osmoregulation in Paramecium: in situ ion gradients permit water to cascade through the cytosol to the contractile vacuole. J. Cell Sci. 115:2339-2348.
  97. Grønlien, H.K., C. Stock, M.S.Aihara, R.D. Allen and Y. Naitoh. 2002. Relationship between the membrane potential of the contractile vacuole complex and its osmoregulatory activity in Paramecium multimicronucleatum. J. Exp. Biol. 205:3261-3270.
  98. Stock, C., H.K. Grønlien and R.D. Allen. 2002. The ionic composition of the contractile vacuole fluid of Paramecium mirrors ion transport across the plasma membrane. Eur. J. Cell Biol. 81:505-515.
  99. Tani, T., T. Tominaga, R.D. Allen and Y. Naitoh. 2002. Development of periodic tension in the contractile vacuole complex membrane of Paramecium governs its membrane dynamics. Cell Biol. Int. 26:853-860. Review.
  100. Iwamoto, M., R.D. Allen and Y. Naitoh. 2003. Hypo-osmotic or Ca2+-rich external conditions trigger extra contractile vacuole complex generation in Paramecium multimicronucleatum. J. Exp. Biol. 206:4467-4473.
  101. Iwamoto, M. and R.D. Allen. 2004. Uptake and rapid transfer of fluorescent ceramide analogues to acidosomes (late endosomes) in Paramecium. J. Histochem. Cytochem. 52:557-565.
  102. Iwamoto, M., K. Sugino, R.D. Allen and Y. Naitoh. 2005. Cell volume control in Paramecium: factors that activate the control mechanisms. J. Exp. Biol. 208:523-537. (Cover)
  103. Sugino, K., T. Tominaga, R.D. Allen and Y. Naitoh. 2005. Electrical properties and fusion dynamics of in vitro membrane vesicles derived from separate parts of the contractile vacuole complex of Paramecium multimicronucleatum. J. Exp. Biol. 208:3957-3969. (Cover)
  104. Fok, A.K., M. Ishida, M.S. Aihara and R.D. Allen. 2008. Calmodulin localization and its effects on endocytic and phagocytic membrane trafficking in Paramecium multimicronucleatum. J. Eukaryot. Microbiol. 55:481-491.
  105. Allen, R.D., T. Tominaga, Y. Naitoh. 2009. The contractile vacuole complex and cell volume control in protozoa. In Osmotic and Ionic Regulation: Cells and Animals, D. H. Evans, ed., CRC Press, Boca Raton, FL., pp 69-105. Review.