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Immunofluorescent pictures of Tetrahymena labeled with an
antibody to the V-ATPase of Dictyostelium discoideum provided
by Dr. T. L. Steck, University of Chicago. a. This polyclonal
antibody was the same as that used in Fok et al., J. Cell Sci.
108:3163-3170, 1995, to label the decorated tubules of
Paramecium. The decorated tubules form a striated cap over the
posterior end of the cell but leave the CV pore and the CV itself
unlabeled. b. During division the cap is reduced in size in the
opisthe while in figure c a new field of decorated tubules
appears near the equatorial region of the dividing cell near the
posterior end of the proter. d. Both decorated-tubule regions
grow in size and in intensity as division proceeds. Fluorescent
micrographs taken by M. Aihara on 12/6/91 and 1/3/92. Unpublished
results. Bars = 20µm.
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