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Paramecium and Other Ciliates: Richard Allen's Image Collection

Chapter 6: Paramecium multimicronucleatum - Digestive Vacuoles

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Fig. 1: accumulation of acidosomes and discoidal vesicles at cytopharynx
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Fig. 2: QF-DE of E-face of the cytopharynx membrane
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Fig. 3: QF-DE of acidosomes docked at NDV
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Fig. 4: DV-I with HRP label surrounded by HRP-free acidosomes
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Fig. 5: HRP in carrier vesicles dock at acidosomes that are docked at nascent vacuole
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Fig. 6: QF-DE of acidosomes docked at a phagosome
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Fig. 7: QF-DE of internal details of acidosomes lying next to E-face of phagosome
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Fig. 8a: antigen B2 is enriched on DV-I phagosomes
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Fig. 8b: antigen B3 is enriched on DV-II phagoacidosomes
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Fig. 8c: antigen B5 is enriched on DV-III phagolysosomes
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Fig. 9: immunogold label specific for B2 antigen on DV-I membrane
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Fig. 10: immunogold label specific for Q2 antigen on DV-I membrane
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Fig. 11: immunogold label specific for Q2 antigen on discoidal vesicles
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Fig. 12: fusion of acidosomes with phagosome membrane
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Fig. 13: immunogold label specific for D6 antigen on acidosomes
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Fig. 14: transfer of L1 antigen from acidosome to DV-I to form DV-II
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Fig. 15: detail of acidosome docking sites at DV-I
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Fig. 16: DV-I membrane is removed as tubules labeled with Q2 antigen
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Fig. 17: DV-II membrane like acidosomes has L1 antigen
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Fig. 18: drawing summarizing membrane replacement
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Fig. 19: acid phosphatase is not found in acidosomes
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Fig. 20: acridine orange shows acidic compartments in living Paramecium
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Fig. 21: acidosomes have A4 antigen which is part of V-ATPase
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Fig. 22: QF-DE detail of acidosome’s fractured membrane
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Fig. 23: QF-DE of acidosome lumen and membrane indentation
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Fig. 24: freeze fractured acidosomes showing indentatiion necks
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Fig. 25: microfilaments associated with acidosomes
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Fig. 26: buildup of microfilaments around acidosome fusion sites
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Fig. 27: bulbous blebs form at acidosome fusion sites
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Fig. 28: phagosome becoming a phagoacidosome
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Fig. 29: QF-DE of development of acidosomes
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Fig. 30: QF-DE of carrier vesicles docked at an acidosome
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Fig. 31: QF-DE shows evidence of carrier vesicle fusion with acidosomes
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Fig. 32: effects of cytochalasin B on actin filaments around DV-I
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Fig. 33: cytochalasin B blocks DV release from cytopharynx
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Fig. 34: a DV-II is surrounded by secondary lysosomes
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Fig. 35: electric shock treatment leads to HRP in secondary lysosomes
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Fig. 36: QF-DE of secondary lysosome near DV-III
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Fig. 37: QF-DE of lysosome lumens show paracrystalline sheets
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Fig. 38: lysosome with paracrystalline material and polysaccharide coat
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Fig. 39: large lysosome in stationary phase cell
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Fig. 40: lysosomes docked on DV-II or phagoacidosome
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Fig. 41: QF-DE of lysosomes docked at DV-II
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Fig. 42: immunogold label specific for L1 antigen on DV-II
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Fig. 43: foci of acid phosphatase in secondary lysosomes docked at DV-II
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Fig. 44: primary lysosomes arising from Golgi stack
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Fig. 45: transfer of lysosome content to phagolysosome
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Fig. 46: phagolysosome shortly after lysosome fusion
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Fig. 47: detail of early phagolysosome membrane
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Fig. 48: freeze fracture of DV-III membrane
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Fig. 49: freeze fracture images of membranes of vesicles compared with DV membranes
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Fig. 50: freeze fracture of early DV-III membrane surrounded by some lysosomes and many smaller vesicles
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Fig. 51: detail of fractured early DV-III membrane
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Fig. 52: membrane glycocalyx and paracrystalline material in DV-III
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Fig. 53: thin section comparison of vesicle and DV membranes
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Fig. 54: QF-DE of paracrystalline sheets in DV-III
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Fig. 55: immunogold label for L1 antigen in 15-18 min DV
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Fig. 56: lysosomal membrane retrieval following digestion
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Fig. 57: detail of membrane retrieval from late DV-III
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Fig. 58: acid phosphatase is present in tubules retrieved from late DV-III
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Fig. 59: summary drawing of the digestive vacuole system in Paramecium
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Fig. 60: "Love"